LAT antibody (AA 29-217)

Catalog No. ABIN8006250

The Rabbit Polyclonal anti-LAT antibody is suitable to detect LAT in samples from Mouse and Rat. It has been validated for WB, ELISA, IHC and FACS.

Quick Overview for LAT antibody (AA 29-217) (ABIN8006250)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-LAT Antibody

Binding Specificity: AA 29-217

Purpose: Anti-Lat Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Mouse Lat shares 68.4%,93.1% amino acid (aa) sequence identity with human,rat Lat,respectively.

Characteristics: Anti-Lat Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Lat recombinant protein (Position: R29-E217). Mouse Lat shares 68.4% and 93.1% amino acid (aa) sequence identity with human and rat Lat, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: Lat

Background:

Background: The Linker for activation of T cells, also known as linker of activated T cells or LAT, is a protein involved in the T-cell antigen receptor signal transduction pathway which in humans is encoded by the LAT gene. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: linker for activation of T cells

Molecular Weight: 38 kDa

Gene ID: 16797

UniProt: O54957

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway