MDH2 antibody (AA 9-223)

Catalog No. ABIN8007526

The Mouse Monoclonal anti-MDH2 antibody (Clone 5D8C1) (ABIN8007526) specifically detects MDH2 in WB, IHC, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for MDH2 antibody (AA 9-223) (ABIN8007526)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Clone: 5D8C1

Product Details anti-MDH2 Antibody

Binding Specificity: AA 9-223

Purpose: Anti-MDH2 Antibody (monoclonal, 5D8C1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human MDH2 shares 97.7%,98.1% amino acid (aa) sequence identity with mouse,rat MDH2,respectively.

Characteristics: Anti-MDH2 Antibody.(monoclonal, 5D8C1)-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MDH2 recombinant protein (Position: A9-L223). Human MDH2 shares 97.7% and 98.1% amino acid (aa) sequence identity with mouse and rat MDH2, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Alternative Name: MDH2

Background:

Background: Malate dehydrogenase, mitochondrial also known as malate dehydrogenase 2 is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: malate dehydrogenase 2

Molecular Weight: 36 kDa

Gene ID: 4191

UniProt: P40926