ME3 antibody (AA 17-585)

Catalog No. ABIN8007614

This Rabbit Polyclonal antibody specifically detects ME3 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for ME3 antibody (AA 17-585) (ABIN8007614)

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ME3 Antibody

Binding Specificity: AA 17-585

Purpose: Anti-ME3 Antibody

Predicted Reactivity: Human ME3 shares 95.8% amino acid (aa) sequence identity with mouse ME3.

Characteristics: Anti-ME3 Antibody. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ME3 recombinant protein (Position: R17-D585). Human ME3 shares 95.8% amino acid (aa) sequence identity with mouse ME3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ME3

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Alternative Name: ME3

Background:

Background: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Gene Full Name: malic enzyme 3

Molecular Weight: 67 kDa

Gene ID: 10873

UniProt: Q16798

Pathways: Warburg Effect