MDC1 antibody (AA 534-626)

Catalog No. ABIN8008450

The Rabbit Polyclonal anti-MDC1 antibody (ABIN8008450) specifically detects MDC1 in IHC, ELISA, IF, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for MDC1 antibody (AA 534-626) (ABIN8008450)

Target: MDC1 (Mdc1 (MDC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-MDC1 Antibody

Binding Specificity: AA 534-626

Purpose: Anti-MDC1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MDC1 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MDC1 recombinant protein (Position: A534-Q626).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1

Background:

Background: Mediator of DNA damage checkpoint protein 1 is a 2080 amino acid long protein that in humans is encoded by the MDC1 gene. The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Gene Full Name: mediator of DNA damage checkpoint 1

Molecular Weight: 50 kDa

Gene ID: 9656

UniProt: Q14676