MBD3 antibody (C-Term)

Catalog No. ABIN8009594

This Rabbit Polyclonal antibody specifically detects MBD3 in WB, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MBD3 antibody (C-Term) (ABIN8009594)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-MBD3 Antibody

Binding Specificity: C-Term

Purpose: Anti-MBD3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human MBD3 shares 100% amino acid (aa) sequence identity with mouse MBD3.

Characteristics: Anti-MBD3 Antibody. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD3. Human MBD3 shares 100% amino acid (aa) sequence identity with mouse MBD3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background:

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene.

Gene Full Name: methyl-CpG binding domain protein 3

Molecular Weight: 33 kDa

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding