MBD4 antibody (AA 238-534) (Biotin)

Catalog No. ABIN8009606

This Rabbit Polyclonal antibody specifically detects MBD4 in WB, ELISA and IHC. It exhibits reactivity toward Mouse and Rat.

Quick Overview for MBD4 antibody (AA 238-534) (Biotin) (ABIN8009606)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MBD4 Antibody (Biotin)

Binding Specificity: AA 238-534

Purpose: Anti-Mbd4 Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Mouse Mbd4 shares 69.7% amino acid (aa) sequence identity with human MBD4.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Mbd4 recombinant protein (Position: R238-D534). Mouse Mbd4 shares 69.7% amino acid (aa) sequence identity with human MBD4.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: Mbd4

Background:

Background: Methyl-CpG-binding domain protein 4 is a protein that in humans is encoded by the MBD4 gene. It is mapped to 3q21.3. The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: methyl-CpG binding domain protein 4

Gene ID: 17193

UniProt: Q9Z2D7

Pathways: DNA Damage Repair