MBD4 antibody (AA 238-534)

Catalog No. ABIN8009616

The Rabbit Polyclonal anti-MBD4 antibody is suitable to detect MBD4 in samples from Mouse and Rat. It has been validated for WB, FACS and ELISA.

Quick Overview for MBD4 antibody (AA 238-534) (ABIN8009616)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Grade: Carrier-free

Product Details anti-MBD4 Antibody

Binding Specificity: AA 238-534

Purpose: Anti-Mbd4 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Mouse Mbd4 shares 69.7% amino acid (aa) sequence identity with human MBD4.

Characteristics: Anti-Mbd4 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Mbd4 recombinant protein (Position: R238-D534). Mouse Mbd4 shares 69.7% amino acid (aa) sequence identity with human MBD4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: Mbd4

Background:

Background: Methyl-CpG-binding domain protein 4 is a protein that in humans is encoded by the MBD4 gene. It is mapped to 3q21.3. The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: methyl-CpG binding domain protein 4

Molecular Weight: 66 kDa

Gene ID: 17193

UniProt: Q9Z2D7

Pathways: DNA Damage Repair