Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody (Fluoro488)

Catalog No. ABIN8010797

This Rabbit Polyclonal antibody specifically detects Mitochondrially Encoded NADH Dehydrogenase 4 in FACS. It exhibits reactivity toward Human.

Quick Overview for Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody (Fluoro488) (ABIN8010797)

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Fluoro488

Application: Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 10-459

Purpose: Anti-MT-ND4 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Alternative Name: MT-ND4

Background:

Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Gene Full Name: NADH dehydrogenase subunit 4

Gene ID: 4538

UniProt: P03905