MPG antibody (C-Term) (Fluoro550)

Catalog No. ABIN8013176

This Rabbit Polyclonal antibody specifically detects MPG in FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MPG antibody (C-Term) (Fluoro550) (ABIN8013176)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPG antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Product Details anti-MPG Antibody (Fluoro550)

Binding Specificity: C-Term

Purpose: Anti-MPG Antibody Fluoro550 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by two amino acids,and from related rat sequence by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MPG, different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: MPG

Background:

Background: MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Gene Full Name: N-methylpurine DNA glycosylase

Sequence Similarities: Belongs to the DNA glycosylase MPG family.

Gene ID: 4350

UniProt: P29372

Pathways: DNA Damage Repair