NEFM antibody (AA 125-916)

Catalog No. ABIN8014734

The Rabbit Polyclonal anti-NEFM antibody is suitable to detect NEFM in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA, IF, FACS and IP.

Quick Overview for NEFM antibody (AA 125-916) (ABIN8014734)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-NEFM Antibody

Binding Specificity: AA 125-916

Purpose: Anti-NF-M/NEFM Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human NF-M,NEFMshares 72.3%,72% amino acid (aa) sequence identity with mouse,rat NF-M,NEFM,respectively.

Characteristics: Anti-NF-M/NEFM Antibody. Tested in WB, IHC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NF-M/NEFM recombinant protein (Position: Q125-D916). Human NF-M/NEFMshares 72.3% and 72% amino acid (aa) sequence identity with mouse and rat NF-M/NEFM, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunoprecipitation, 0.5-2 μg/mL, Human Immunofluorescence, 5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: NEFM

Background:

Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Full Name: neurofilament medium chain

Molecular Weight: 160 kDa

Gene ID: 4741

UniProt: P07197

Pathways: Brown Fat Cell Differentiation