Nibrin antibody (C-Term) (HRP)

Catalog No. ABIN8015423

This Mouse Monoclonal antibody specifically detects Nibrin in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Nibrin antibody (C-Term) (HRP) (ABIN8015423)

Target: Nibrin (NBN)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nibrin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 5D7A1

Product Details anti-Nibrin Antibody (HRP)

Binding Specificity: C-Term

Purpose: Anti-p95 NBS1 Antibody (monoclonal, 5D7A1) HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by three amino acids,and from the related rat sequence by five amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p95 NBS1, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG2a

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: NBN

Background:

Background: p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50(N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).

Gene Full Name: nibrin

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response