Nibrin antibody (C-Term)

Catalog No. ABIN8015427

The Mouse Monoclonal anti-Nibrin antibody is suitable to detect Nibrin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for Nibrin antibody (C-Term) (ABIN8015427)

Target: Nibrin (NBN)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 5D7A1

Product Details anti-Nibrin Antibody

Binding Specificity: C-Term

Purpose: Anti-p95 NBS1 Antibody (monoclonal, 5D7A1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by three amino acids,and from the related rat sequence by five amino acids.

Characteristics: Anti-p95 NBS1 Antibody.(monoclonal, 5D7A1)-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p95 NBS1, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: NBN

Background:

Background: p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50(N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).

Gene Full Name: nibrin

Molecular Weight: 95 kDa

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response