NNT antibody (AA 44-350)

Catalog No. ABIN8015493

The Rabbit Polyclonal anti-NNT antibody is suitable to detect NNT in samples from Human, Mouse, Rat, Pig and Monkey. It has been validated for WB, ELISA, FACS, IF and ICC.

Quick Overview for NNT antibody (AA 44-350) (ABIN8015493)

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Reactivity: Human, Mouse, Rat, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NNT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-NNT Antibody

Binding Specificity: AA 44-350

Purpose: Anti-NNT Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NNT Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Pig.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NNT recombinant protein (Position: C44-A350).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat, Pig Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NNT

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Alternative Name: NNT

Background:

Background: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD (H) and NADP (+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

Gene Full Name: nicotinamide nucleotide transhydrogenase

Molecular Weight: 114 kDa

Gene ID: 23530

UniProt: Q13423

Pathways: Proton Transport