ORC2 antibody (AA 130-493)

Catalog No. ABIN8018379

The Rabbit Polyclonal anti-ORC2 antibody is suitable to detect ORC2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS, IF and ICC.

Quick Overview for ORC2 antibody (AA 130-493) (ABIN8018379)

Target: ORC2 (Origin Recognition Complex Subunit 2 (ORC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ORC2 Antibody

Binding Specificity: AA 130-493

Purpose: Anti-ORC2 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human ORC2 shares 80.2%,79.4% amino acid (aa) sequence identity with mouse,rat ORC2,respectively.

Characteristics: Anti-ORC2 Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ORC2 recombinant protein (Position: E130-R493). Human ORC2 shares 80.2% and 79.4% amino acid (aa) sequence identity with mouse and rat ORC2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ORC2

Target: ORC2 (Origin Recognition Complex Subunit 2 (ORC2))

Alternative Name: ORC2

Background:

Background: Origin recognition complex subunit 2 is a protein that is encoded by the ORC2 (ORC2L) gene in humans. The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate.

Gene Full Name: origin recognition complex subunit 2

Molecular Weight: 74 kDa

Gene ID: 4999

UniProt: Q13416