Osteoactivin antibody (AA 164-564)

Catalog No. ABIN8018490

The Rabbit Polyclonal anti-Osteoactivin antibody is suitable to detect Osteoactivin in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS, ICC and IF.

Quick Overview for Osteoactivin antibody (AA 164-564) (ABIN8018490)

Target: Osteoactivin (GPNMB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteoactivin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Osteoactivin Antibody

Binding Specificity: AA 164-564

Purpose: Anti-GPNMB/Gpnmb Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GPNMB/Gpnmb.Antibody-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Osteoactivin

Target: Osteoactivin (GPNMB)

Alternative Name: Gpnmb

Background:

Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3, 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: glycoprotein (transmembrane) nmb

Molecular Weight: 64 kDa

Gene ID: 93695

UniProt: Q99P91