PPHLN1 antibody (Middle Region)

Catalog No. ABIN8020549

The Rabbit Polyclonal anti-PPHLN1 antibody (ABIN8020549) specifically detects PPHLN1 in WB, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PPHLN1 antibody (Middle Region) (ABIN8020549)

Target: PPHLN1 (Periphilin 1 (PPHLN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPHLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PPHLN1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Periphilin 1/PPHLN1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Periphilin 1/PPHLN1 Antibody. Tested in WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Periphilin 1/PPHLN1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPHLN1

Target: PPHLN1 (Periphilin 1 (PPHLN1))

Alternative Name: PPHLN1

Background:

Background: The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Full Name: periphilin 1

Molecular Weight: 60 kDa

Gene ID: 51535

UniProt: Q8NEY8