Phenylalanine Hydroxylase antibody (AA 71-208)

Catalog No. ABIN8021075

This Rabbit Polyclonal antibody specifically detects Phenylalanine Hydroxylase in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Phenylalanine Hydroxylase antibody (AA 71-208) (ABIN8021075)

Target: Phenylalanine Hydroxylase

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phenylalanine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Phenylalanine Hydroxylase Antibody

Binding Specificity: AA 71-208

Purpose: Anti-PAH Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1%,88.4% amino acid (aa) sequence identity with mouse,rat PAH,respectively.

Characteristics: Anti-PAH Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Phenylalanine Hydroxylase

Target: Phenylalanine Hydroxylase

Alternative Name: PAH

Background:

Background: Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Gene Full Name: phenylalanine hydroxylase

Molecular Weight: 45 kDa

Gene ID: 5053

UniProt: P00439