PEMT antibody (C-Term)

Catalog No. ABIN8021232

The Rabbit Polyclonal anti-PEMT antibody is suitable to detect PEMT in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for PEMT antibody (C-Term) (ABIN8021232)

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PEMT Antibody

Binding Specificity: C-Term

Purpose: Anti-PEMT Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PEMT shares 94.7%,84.2% amino acid (aa) sequence identity with mouse,rat PEMT,respectively.

Characteristics: Anti-PEMT Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PEMT. Human PEMT shares 94.7% and 84.2% amino acid (aa) sequence identity with mouse and rat PEMT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PEMT

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Alternative Name: PEMT

Background:

Background: Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Gene Full Name: phosphatidylethanolamine N-methyltransferase

Molecular Weight: 17 kDa

Gene ID: 10400

UniProt: Q9UBM1