PEPCK antibody (AA 1-640) (Fluoro594)

Catalog No. ABIN8021595

The Mouse Monoclonal anti-PEPCK antibody is suitable to detect PEPCK in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for PEPCK antibody (AA 1-640) (Fluoro594) (ABIN8021595)

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PEPCK antibody is conjugated to Fluoro594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 3F7

Product Details anti-PEPCK Antibody (Fluoro594)

Binding Specificity: AA 1-640

Purpose: Anti-PCK2 Antibody (monoclonal, 3F7) Fluoro594 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PCK2 recombinant protein (Position: M1-M640).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PEPCK

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Alternative Name: PCK2

Background:

Background: Phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2, PEPCK-M), is an isozyme of phosphoenolpyruvate carboxykinase (PCK, PEPCK) that in humans is encoded by the PCK2 gene. It is mapped to 14q11.2-q12. This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Gene Full Name: phosphoenolpyruvate carboxykinase 2, mitochondrial

Gene ID: 5106

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis