PIK3R5 antibody (AA 170-864)

Catalog No. ABIN8021922

The Rabbit Polyclonal anti-PIK3R5 antibody is suitable to detect PIK3R5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for PIK3R5 antibody (AA 170-864) (ABIN8021922)

Target: PIK3R5 (Phosphoinositide-3-Kinase, Regulatory Subunit 5 (PIK3R5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PIK3R5 Antibody

Binding Specificity: AA 170-864

Purpose: Anti-PIK3R5 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PIK3R5 shares 83.9% amino acid (aa) sequence identity with mouse PIK3R5.

Characteristics: Anti-PIK3R5 Antibody. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PIK3R5 recombinant protein (Position: E170-L864). Human PIK3R5 shares 83.9% amino acid (aa) sequence identity with mouse PIK3R5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIK3R5

Target: PIK3R5 (Phosphoinositide-3-Kinase, Regulatory Subunit 5 (PIK3R5))

Alternative Name: PIK3R5

Background:

Background: Phosphoinositide 3-kinase regulatory subunit 5 is an enzyme that in humans is encoded by the PIK3R5 gene. Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found.

Gene Full Name: phosphoinositide-3-kinase regulatory subunit 5

Molecular Weight: 97 kDa

Gene ID: 23533

UniProt: Q8WYR1

Pathways: PI3K-Akt Signaling, Inositol Metabolic Process, Hepatitis C, VEGF Signaling