PLCD1 antibody (AA 14-549)

Catalog No. ABIN8022168

The Rabbit Polyclonal anti-PLCD1 antibody is suitable to detect PLCD1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for PLCD1 antibody (AA 14-549) (ABIN8022168)

Target: PLCD1 (phospholipase C, delta 1 (PLCD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLCD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PLCD1 Antibody

Binding Specificity: AA 14-549

Purpose: Anti-PLCD1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PLCD1 shares 92.2%,92.5% amino acid (aa) sequence identity with mouse,rat PLCD1,respectively.

Characteristics: Anti-PLCD1 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLCD1 recombinant protein (Position: Q14-R549). Human PLCD1 shares 92.2% and 92.5% amino acid (aa) sequence identity with mouse and rat PLCD1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PLCD1

Target: PLCD1 (phospholipase C, delta 1 (PLCD1))

Alternative Name: PLCD1

Background:

Background: This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: phospholipase C delta 1

Molecular Weight: 86 kDa

Gene ID: 5333

UniProt: P51178

Pathways: WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process