PLCE1 antibody (AA 4-735)

Catalog No. ABIN8022179

This Rabbit Polyclonal antibody specifically detects PLCE1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PLCE1 antibody (AA 4-735) (ABIN8022179)

Target: PLCE1 (Phospholipase C, epsilon 1 (PLCE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLCE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-PLCE1 Antibody

Binding Specificity: AA 4-735

Purpose: Anti-PLCE1 Antibody

Predicted Reactivity: Human PLCE1 shares 74.6%,73.6% amino acid (aa) sequence identity with mouse,rat PLCE1,respectively.

Characteristics: Anti-PLCE1 Antibody. Tested in WB, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLCE1 recombinant protein (Position: E4-E735). Human PLCE1 shares 74.6% and 73.6% amino acid (aa) sequence identity with mouse and rat PLCE1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PLCE1

Target: PLCE1 (Phospholipase C, epsilon 1 (PLCE1))

Alternative Name: PLCE1

Background:

Background: Phospholipase C epsilon 1 (PLCE1) is an enzyme that in humans is encoded by the PLCE1 gene. This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome, characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Full Name: phospholipase C epsilon 1

Molecular Weight: 290 kDa

Gene ID: 51196

UniProt: Q9P212

Pathways: EGFR Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling