POLE2 antibody (AA 46-483)

Catalog No. ABIN8024119

The Rabbit Polyclonal anti-POLE2 antibody (ABIN8024119) specifically detects POLE2 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for POLE2 antibody (AA 46-483) (ABIN8024119)

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-POLE2 Antibody

Binding Specificity: AA 46-483

Purpose: Anti-POLE2 Antibody

Predicted Reactivity: Human POLE2 shares 99.1% amino acid (aa) sequence identity with mouse POLE2.

Characteristics: Anti-POLE2 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLE2 recombinant protein (Position: E46-K483). Human POLE2 shares 99.1% amino acid (aa) sequence identity with mouse POLE2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLE2

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Alternative Name: POLE2

Background:

Background: DNA polymerase epsilon subunit 2 is an enzyme that in humans is encoded by the POLE2 gene. DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.

Gene Full Name: DNA polymerase epsilon 2, accessory subunit

Gene ID: 5427

UniProt: P56282

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA