POLH antibody (AA 157-361) (Fluoro594)

Catalog No. ABIN8024136

The Rabbit Polyclonal anti-POLH antibody is suitable to detect POLH in samples from Human and Rat. It has been validated for FACS.

Quick Overview for POLH antibody (AA 157-361) (Fluoro594) (ABIN8024136)

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLH antibody is conjugated to Fluoro594

Application: Flow Cytometry (FACS)

Product Details anti-POLH Antibody (Fluoro594)

Binding Specificity: AA 157-361

Purpose: Anti-DNA polymerase eta/POLH Antibody Fluoro594 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLH recombinant protein (Position: A157-R361). Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Alternative Name: POLH

Background:

Background: DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-ed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: DNA polymerase eta

Gene ID: 5429

UniProt: Q9Y253

Pathways: DNA Damage Repair