PAWR antibody (AA 160-255)

Catalog No. ABIN8025296

This Rabbit Polyclonal antibody specifically detects PAWR in WB, ELISA, FACS, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PAWR antibody (AA 160-255) (ABIN8025296)

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAWR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PAWR Antibody

Binding Specificity: AA 160-255

Purpose: Anti-PAWR Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PAWR shares 82.3%,81.2% amino acid (aa) sequence identity with mouse,rat PAWR,respectively.

Characteristics: Anti-PAWR Antibody. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PAWR recombinant protein (Position: R160-D255). Human PAWR shares 82.3% and 81.2% amino acid (aa) sequence identity with mouse and rat PAWR, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PAWR

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Alternative Name: PAWR

Background:

Background: PRKC apoptosis WT1 regulator protein, or Prostate apoptosis response-4, is a tumor-suppressor protein coded for in the human by the PAWR gene, that induces apoptosis in cancer cells, but not in normal cells. This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.

Gene Full Name: pro-apoptotic WT1 regulator

Molecular Weight: 41 kDa

Gene ID: 5074

UniProt: Q96IZ0