PRKDC antibody (N-Term)

Catalog No. ABIN8027720

This Rabbit Polyclonal antibody specifically detects PRKDC in WB. It exhibits reactivity toward Human.

Quick Overview for PRKDC antibody (N-Term) (ABIN8027720)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-PRKDC Antibody

Binding Specificity: N-Term

Purpose: Anti-DNA PKcs/PRKDC Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DNA PKcs/PRKDC Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DNA PKcs.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: PRKDC

Background:

Background: PRKDC (Protein Kinase DNA-Activated Catalytic Subunit), also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al. (1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller (1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al. (1999) demonstrated that the PRKDC protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.

Gene Full Name: protein kinase, DNA-activated, catalytic subunit

Sequence Similarities: Belongs to the PI3/PI4-kinase family.

Molecular Weight: 460 kDa

Gene ID: 5591

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response