PYCARD antibody (AA 3-193)

Catalog No. ABIN8029176

The Rabbit Polyclonal anti-PYCARD antibody is suitable to detect PYCARD in samples from Mouse and Rat. It has been validated for WB, ELISA, IHC and FACS.

Quick Overview for PYCARD antibody (AA 3-193) (ABIN8029176)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PYCARD Antibody

Binding Specificity: AA 3-193

Purpose: Anti-TMS1/ASC/Pycard Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TMS1/ASC/Pycard Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse TMS1/ASC/PYCARD recombinant protein (Position: R3-S193).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: Pycard

Background:

Background: PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm, however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: PYD and CARD domain containing

Molecular Weight: 22-25 kDa

Gene ID: 66824

UniProt: Q9EPB4

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome