PYCARD antibody (AA 24-193) (Biotin)

Catalog No. ABIN8029177

The Biotin-conjugated Rabbit Polyclonal anti-PYCARD antibody (ABIN8029177) specifically detects PYCARD in WB, ELISA and IHC. The antibody is reactive with Mouse samples.

Quick Overview for PYCARD antibody (AA 24-193) (Biotin) (ABIN8029177)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PYCARD Antibody (Biotin)

Binding Specificity: AA 24-193

Purpose: Anti-ASC/TMS1/Pycard Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse ASC/TMS1/Pycard recombinant protein (Position: K24-S193).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: Pycard

Background:

Background: PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm, however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: PYD and CARD domain containing

Gene ID: 66824

UniProt: Q9EPB4

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome