RAD51 Homolog B antibody (AA 22-384)

Catalog No. ABIN8030105

This Rabbit Polyclonal antibody specifically detects RAD51 Homolog B in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for RAD51 Homolog B antibody (AA 22-384) (ABIN8030105)

Target: RAD51 Homolog B (Rad51B)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 Homolog B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-RAD51 Homolog B Antibody

Binding Specificity: AA 22-384

Purpose: Anti-RAD51L1/RAD51B Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human RAD51L1,RAD51B shares 85.8% amino acid (aa) sequence identity with mouse RAD51L1,RAD51B.

Characteristics: Anti-RAD51L1/RAD51B Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAD51L1/RAD51B recombinant protein (Position: H22-F384). Human RAD51L1/RAD51B shares 85.8% amino acid (aa) sequence identity with mouse RAD51L1/RAD51B.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD51 Homolog B

Target: RAD51 Homolog B (Rad51B)

Alternative Name: RAD51B

Background:

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata.

Gene Full Name: RAD51 paralog B

Molecular Weight: 42 kDa

Gene ID: 5890

UniProt: O15315

Pathways: DNA Damage Repair