GFR antibody (AA 164-574)

Catalog No. ABIN8030469

The Rabbit Polyclonal anti-GFR antibody is suitable to detect GFR in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for GFR antibody (AA 164-574) (ABIN8030469)

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-GFR Antibody

Binding Specificity: AA 164-574

Purpose: Anti-RAPGEF5 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human RAPGEF5 shares 95.6%,96.4% amino acid (aa) sequence identity with mouse,rat RAPGEF5,respectively.

Characteristics: Anti-RAPGEF5 Antibody. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAPGEF5 recombinant protein (Position: E164-H574). Human RAPGEF5 shares 95.6% and 96.4% amino acid (aa) sequence identity with mouse and rat RAPGEF5, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GFR

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Alternative Name: RAPGEF5

Background:

Background: Rap guanine nucleotide exchange factor 5 is a protein that in humans is encoded by the RAPGEF5 gene. Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation.

Gene Full Name: Rap guanine nucleotide exchange factor 5

Molecular Weight: 75 kDa

Gene ID: 9771

UniProt: Q92565

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, Hepatitis C