Retinoblastoma Binding Protein 8 antibody (AA 307-702)

Catalog No. ABIN8031829

The Rabbit Polyclonal anti-Retinoblastoma Binding Protein 8 antibody (ABIN8031829) specifically detects Retinoblastoma Binding Protein 8 in ELISA, WB, IHC, IF, FACS and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Retinoblastoma Binding Protein 8 antibody (AA 307-702) (ABIN8031829)

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma Binding Protein 8 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-Retinoblastoma Binding Protein 8 Antibody

Binding Specificity: AA 307-702

Purpose: Anti-RBBP8 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human RBBP8 shares 68.9%,66.7% amino acid (aa) sequence identity with mouse,rat RBBP8,respectively.

Characteristics: Anti-RBBP8 Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RBBP8 recombinant protein (Position: R307-K702). Human RBBP8 shares 68.9% and 66.7% amino acid (aa) sequence identity with mouse and rat RBBP8, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Retinoblastoma Binding Protein 8

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Alternative Name: RBBP8

Background:

Background: Retinoblastoma-binding protein 8 is a protein that in humans is encoded by the RBBP8 gene. The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind ly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Gene Full Name: RB binding protein 8, endonuclease

Molecular Weight: 130 kDa

Gene ID: 5932

UniProt: Q99708

Pathways: Cell Division Cycle, DNA Damage Repair