RBX1 antibody (C-Term) (Fluoro647)

Catalog No. ABIN8033414

This Rabbit Polyclonal antibody specifically detects RBX1 in FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RBX1 antibody (C-Term) (Fluoro647) (ABIN8033414)

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-RBX1 Antibody (Fluoro647)

Binding Specificity: C-Term

Purpose: Anti-ROC1/RBX1 Antibody Fluoro647 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse sequence.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ROC1, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1

Background:

Background: RING-box protein 1, also known as ROC1, is a protein that in humans is encoded by the RBX1 gene. This gene is mapped to chromosome 22q13.2 based on an alignment of the RBX1 sequence with the genomic sequence. ROC1 is recruited by cullin-1 to form a quaternary SCF (HOS)-ROC1 holoenzyme (with SKP1 and the BTRCP homolog HOS). SCF (HOS)-ROC1 binds IKK-beta-phosphorylated I-kappa-B-alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and CDC34. Conclusively, ROC1 plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization.

Gene Full Name: ring-box 1

Gene ID: 9978

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome