RMI2 antibody (C-Term) (Cy3)

Catalog No. ABIN8033439

The Rabbit Polyclonal anti-RMI2 antibody is suitable to detect RMI2 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for RMI2 antibody (C-Term) (Cy3) (ABIN8033439)

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RMI2 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-RMI2 Antibody (Cy3)

Binding Specificity: C-Term

Purpose: Anti-RMI2 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RMI2, which shares 96.3% and 92.6% amino acid (aa) sequence identity with mouse and rat RMI2, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for RMI2

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Alternative Name: RMI2

Background:

Background: RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (see RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted ly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90 % . Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.

Gene Full Name: RecQ mediated genome instability 2

Gene ID: 116028

UniProt: Q96E14

Pathways: DNA Damage Repair