RYK antibody (AA 66-607)

Catalog No. ABIN8034205

The Rabbit Polyclonal anti-RYK antibody (ABIN8034205) specifically detects RYK in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RYK antibody (AA 66-607) (ABIN8034205)

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-RYK Antibody

Binding Specificity: AA 66-607

Purpose: Anti-RYK Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human RYK shares 97.2% amino acid (aa) sequence identity with mouse RYK.

Characteristics: Anti-RYK Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RYK recombinant protein (Position: L66-V607). Human RYK shares 97.2% amino acid (aa) sequence identity with mouse RYK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RYK

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Alternative Name: RYK

Background:

Background: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Full Name: receptor like tyrosine kinase

Molecular Weight: 75 kDa

Gene ID: 6259

UniProt: P34925

Pathways: RTK Signaling, WNT Signaling, Regulation of Cell Size