Osteopontin antibody (C-Term)

Catalog No. ABIN8035141

The Rabbit Polyclonal anti-Osteopontin antibody (ABIN8035141) specifically detects Osteopontin in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Osteopontin antibody (C-Term) (ABIN8035141)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-Osteopontin Antibody

Binding Specificity: C-Term

Purpose: Anti-Osteopontin/SPP1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by eight amino acids,and from the related rat sequence by seven amino acids.

Characteristics: Anti-Osteopontin/SPP1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, -ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Background:

Background: Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: secreted phosphoprotein 1

Sequence Similarities: Belongs to the osteopontin family.

Molecular Weight: 66 kDa, 25-55 kDa

Gene ID: 6696

UniProt: P10451

Pathways: Regulation of Cell Size