SQSTM1 antibody (N-Term) (HRP)

Catalog No. ABIN8035798

The HRP-conjugated Rabbit Polyclonal anti-SQSTM1 antibody (ABIN8035798) specifically detects SQSTM1 in WB, IHC and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SQSTM1 antibody (N-Term) (HRP) (ABIN8035798)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SQSTM1 Antibody (HRP)

Binding Specificity: N-Term

Purpose: Anti-SQSTM1/p62 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background:

Background: SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Gene Full Name: sequestosome 1

Sequence Similarities: Contains 1 PB1 domain.

Gene ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy