SMPD3 antibody (AA 184-553) (Fluoro550)

Catalog No. ABIN8041677

The Rabbit Polyclonal anti-SMPD3 antibody is suitable to detect SMPD3 in samples from Human. It has been validated for FACS.

Quick Overview for SMPD3 antibody (AA 184-553) (Fluoro550) (ABIN8041677)

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD3 antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Product Details anti-SMPD3 Antibody (Fluoro550)

Binding Specificity: AA 184-553

Purpose: Anti-SMPD3 Antibody Fluoro550 Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human SMPD3 shares 88.9% amino acid (aa) sequence identity with both mouse,rat SMPD3.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SMPD3 recombinant protein (Position: F184-D553). Human SMPD3 shares 88.9% amino acid (aa) sequence identity with both mouse and rat SMPD3.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for SMPD3

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Alternative Name: SMPD3

Background:

Background: Sphingomyelin phosphodiesterase 3 is an enzyme that in humans is encoded by the SMPD3 gene. SMPD3 (Sphingomyelin phosphodiesterase 3, also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91 % aa sequence identity with mouse SMPD3.

Gene Full Name: sphingomyelin phosphodiesterase 3

Gene ID: 55512

UniProt: Q9NY59

Pathways: Hormone Transport