SPO11 antibody (AA 40-326)

Catalog No. ABIN8041961

This Rabbit Polyclonal antibody specifically detects SPO11 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPO11 antibody (AA 40-326) (ABIN8041961)

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPO11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-SPO11 Antibody

Binding Specificity: AA 40-326

Purpose: Anti-SPO11 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human SPO11 shares 83.3% amino acid (aa) sequence identity with mouse SPO11.

Characteristics: Anti-SPO11 Antibody. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPO11 recombinant protein (Position: R40-D326). Human SPO11 shares 83.3% amino acid (aa) sequence identity with mouse SPO11.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPO11

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Alternative Name: SPO11

Background:

Background: Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described.

Gene Full Name: SPO11 initiator of meiotic double strand breaks

Molecular Weight: 70 kDa

Gene ID: 23626

UniProt: Q9Y5K1