TYMS antibody (AA 1-313)

Catalog No. ABIN8046532

The Rabbit Polyclonal anti-TYMS antibody (ABIN8046532) specifically detects TYMS in WB, IF, IHC and ELISA. The antibody is reactive with Human, Rat and Monkey samples.

Quick Overview for TYMS antibody (AA 1-313) (ABIN8046532)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Grade: Carrier-free

Product Details anti-TYMS Antibody

Binding Specificity: AA 1-313

Purpose: Anti-Thymidylate Synthase/TYMS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Thymidylate Synthase/TYMS Antibody. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Thymidylate Synthase/TYMS recombinant protein (Position: M1-V313).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TYMS

Background:

Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy.

Gene Full Name: thymidylate synthetase

Molecular Weight: 36 kDa

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases