TIGAR antibody (AA 1-270)

Catalog No. ABIN8047632

The Rabbit Polyclonal anti-TIGAR antibody is suitable to detect TIGAR in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF, FACS and ICC.

Quick Overview for TIGAR antibody (AA 1-270) (ABIN8047632)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-TIGAR Antibody

Binding Specificity: AA 1-270

Purpose: Anti-TIGAR Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human TIGAR shares 72.8% amino acid (aa) sequence identity with mouse TIGAR.

Characteristics: Anti-TIGAR Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TIGAR recombinant protein (Position: M1-R270). Human TIGAR shares 72.8% amino acid (aa) sequence identity with mouse TIGAR.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background:

Background: This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.

Gene Full Name: TP53 induced glycolysis regulatory phosphatase

Molecular Weight: 30 kDa

Gene ID: 57103

UniProt: Q9NQ88

Pathways: Warburg Effect