TIGAR antibody (AA 1-269)

Catalog No. ABIN8047643

This Rabbit Polyclonal antibody specifically detects TIGAR in WB, ELISA, IHC and IF. It exhibits reactivity toward Mouse and Rat.

Quick Overview for TIGAR antibody (AA 1-269) (ABIN8047643)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-TIGAR Antibody

Binding Specificity: AA 1-269

Purpose: Anti-TIGAR Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Mouse TIGAR shares 72.8% amino acid (aa) sequence identity with human TIGAR.

Characteristics: Anti-TIGAR Antibody. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse TIGAR recombinant protein (Position: M1-H269). Mouse TIGAR shares 72.8% amino acid (aa) sequence identity with human TIGAR.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat< Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunofluorescence, 5 μg/mL, Mouse ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background:

Background: Enables fructose-2,6-bisphosphate 2-phosphatase activity. Involved in positive regulation of pentose-phosphate shunt and response to ischemia. Acts upstream of or within several processes, including fructose 2,6-bisphosphate metabolic process, negative regulation of catabolic process, and positive regulation of cardiac muscle cell apoptotic process. Located in mitochondrion. Is expressed in several structures, including brain, heart, immune system, and liver. Orthologous to human TIGAR (TP53 induced glycolysis regulatory phosphatase).

Gene Full Name: Trp53 induced glycolysis regulatory phosphatase

Molecular Weight: 30 kDa

Gene ID: 319801

UniProt: Q8BZA9

Pathways: Warburg Effect