TP53BP1 antibody (AA 1239-1972)

Catalog No. ABIN8051599

The Rabbit Polyclonal anti-TP53BP1 antibody (ABIN8051599) specifically detects TP53BP1 in IHC, WB, ELISA, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for TP53BP1 antibody (AA 1239-1972) (ABIN8051599)

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53BP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-TP53BP1 Antibody

Binding Specificity: AA 1239-1972

Purpose: Anti-53BP1/TP53BP1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-53BP1/TP53BP1 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human 53BP1/TP53BP1 recombinant protein (Position: H1239-H1972).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TP53BP1

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Alternative Name: TP53BP1

Background:

Background: TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA ed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Gene Full Name: tumor protein p53 binding protein 1

Molecular Weight: 450 kDa

Gene ID: 7158

UniProt: Q12888

Pathways: DNA Damage Repair