UBE2M antibody (AA 25-154) (Biotin)

Catalog No. ABIN8052363

The Rabbit Polyclonal anti-UBE2M antibody is suitable to detect UBE2M in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for UBE2M antibody (AA 25-154) (Biotin) (ABIN8052363)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-UBE2M Antibody (Biotin)

Binding Specificity: AA 25-154

Purpose: Anti-UBC12/UBE2M Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UBC12/UBE2M recombinant protein (Position: K25-Q154).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M

Background:

Background: NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Gene Full Name: ubiquitin conjugating enzyme E2 M

Gene ID: 9040

UniProt: P61081