UCP2 antibody (Middle Region) (Biotin)

Catalog No. ABIN8052807

The Rabbit Polyclonal anti-UCP2 antibody is suitable to detect UCP2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for UCP2 antibody (Middle Region) (Biotin) (ABIN8052807)

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-UCP2 Antibody (Biotin)

Binding Specificity: Middle Region

Purpose: Anti-UCP2 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse,rat sequences by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human UCP2, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP2

Background:

Background: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Gene Full Name: uncoupling protein 2

Gene ID: 7351

UniProt: P55851

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport