RECQL2 antibody (AA 122-240) (Fluoro647)

Catalog No. ABIN8054181

The Rabbit Polyclonal anti-RECQL2 antibody is suitable to detect RECQL2 in samples from Human and Rat. It has been validated for FACS.

Quick Overview for RECQL2 antibody (AA 122-240) (Fluoro647) (ABIN8054181)

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RECQL2 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-RECQL2 Antibody (Fluoro647)

Binding Specificity: AA 122-240

Purpose: Anti-Werner's syndrome helicase WRN/WRN Antibody Fluoro647 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for RECQL2

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Alternative Name: WRN

Background:

Background: Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Gene Full Name: WRN RecQ like helicase

Gene ID: 7486

UniProt: Q14191

Pathways: DNA Damage Repair