WHSC2 antibody (AA 38-482)

Catalog No. ABIN8054490

The Rabbit Polyclonal anti-WHSC2 antibody is suitable to detect WHSC2 in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for WHSC2 antibody (AA 38-482) (ABIN8054490)

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WHSC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-WHSC2 Antibody

Binding Specificity: AA 38-482

Purpose: Anti-NELFA Antibody

Predicted Reactivity: Human NELFA shares 94.6% amino acid (aa) sequence identity with mouse NELFA.

Characteristics: Anti-NELFA Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NELFA recombinant protein (Position: I38-L482). Human NELFA shares 94.6% amino acid (aa) sequence identity with mouse NELFA.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for WHSC2

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Alternative Name: NELFA

Background:

Background: This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93 % sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.

Gene Full Name: negative elongation factor complex member A

Molecular Weight: 57 kDa

Gene ID: 7469

UniProt: Q9H3P2

Pathways: Chromatin Binding