PAPPA antibody

Catalog No. ABIN8065522

The Mouse Monoclonal anti-PAPPA antibody (Clone 9) (ABIN8065522) specifically detects PAPPA in IHC, WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for PAPPA antibody (ABIN8065522)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Grade: Carrier-free

Clone: 9

Product Details anti-PAPPA Antibody

Purpose: Anti-Pregnancy Associated Plasma Protein A (PAPP-A) Antibody

Specificity: ABIN8065522 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. The epitope of ABIN8065522 is different from ABIN8065520 and ABIN8065521. The epitope is located on the PAPP-A subunit of the heterotetramer.

Predicted Reactivity: Human

Purification: Protein A or Protein G purified

Immunogen: Purified human PAPP-A

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 24 months

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: Pregnancy Associated Plasma Protein A

Background: PAPPA, ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1, pappalysin-1, IGF-dependent IGFBP-4 protease, aspecific BCL2 ARE-binding protein 2, differentially placenta 1 expressed protein, insulin-like growth factor-dependent IGF binding protein-4 protease, pregnancy-associated plasma protein A, pappalysin 1,Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. {ECO:0000269|PubMed:10077652, ECO:0000269|PubMed:10913121, ECO:0000269|PubMed:11522292}.,PAPPA,Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome (trisomy 21).

Molecular Weight: 500 kDa (tetramer), 180 kDa (monomer)

Gene ID: 5069

UniProt: Q13219