Recombinant ERCC2 antibody

Catalog No. ABIN8093170

This Rabbit Monoclonal antibody specifically detects ERCC2 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant ERCC2 antibody (ABIN8093170)

Target: ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERCC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 25E62

Product Details anti-ERCC2 Antibody

Purpose: ERCC2 Antibody / DNA excision repair protein ERCC-2 / XPD

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human DNA excision repair protein ERCC-2 was used as the immunogen for the ERCC2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ERCC2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the ERCC2 antibody at -20oC.

Target Details for ERCC2

Target: ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2))

Alternative Name: ERCC2

Background: ERCC2 antibody targets DNA excision repair protein ERCC-2 (ERCC2), also widely known as XPD, a DNA helicase that functions as an essential component of the transcription factor IIH (TFIIH) complex. ERCC2 localizes predominantly to the nucleus, where it participates in nucleotide excision repair and transcription initiation. As a helicase, ERCC2 unwinds DNA around sites of damage, enabling recognition and removal of bulky DNA lesions caused by ultraviolet radiation and chemical mutagens. This activity places ERCC2 at the intersection of DNA repair and transcriptional regulation, two processes critical for maintaining genomic stability.

Functionally, ERCC2 plays a central role in the nucleotide excision repair pathway by facilitating local DNA strand separation at damaged sites. Within the TFIIH complex, ERCC2 works in coordination with other helicase and structural subunits to verify DNA damage and recruit downstream repair factors. In addition to its repair function, ERCC2 contributes to transcription initiation by assisting RNA polymerase II in promoter clearance. Through these dual roles, ERCC2 helps ensure that DNA integrity is preserved while allowing regulated gene expression to proceed. An ERCC2 antibody supports studies examining DNA damage responses and transcription-coupled repair mechanisms.

ERCC2 expression and activity are tightly regulated in proliferating and metabolically active cells, reflecting the need for efficient DNA repair during replication and transcription. Changes in ERCC2 abundance or function can influence cellular sensitivity to DNA-damaging agents and alter transcriptional fidelity. Analysis of ERCC2 localization and expression provides insight into how cells coordinate repair and transcription under normal and stress conditions, making ERCC2 a valuable marker for nuclear DNA maintenance pathways.

From a biological and disease-relevance perspective, ERCC2 has been extensively studied in the context of inherited DNA repair disorders and cancer biology. Mutations in ERCC2 are associated with conditions characterized by defective nucleotide excision repair, including xeroderma pigmentosum and related syndromes. In oncology research, ERCC2 status has been linked to cellular responses to DNA-damaging chemotherapy and environmental stress. Understanding ERCC2 expression and regulation therefore contributes to broader investigations of genome stability, mutagen sensitivity, and disease-associated DNA repair defects.

At the molecular level, ERCC2 is encoded by the ERCC2 gene and produces a nuclear protein of approximately 73 kDa. The protein contains conserved helicase motifs required for ATP binding and DNA unwinding activity. ERCC2 function depends on its incorporation into the TFIIH complex and interactions with partner proteins that regulate repair and transcriptional activity. An ERCC2 antibody supports research applications focused on DNA repair pathways, transcriptional regulation, and genomic maintenance, with NSJ Bioreagents providing reagents intended for research use.

UniProt: P18074

Pathways: DNA Damage Repair