Recombinant FOLR1 antibody (Extracellular)

Catalog No. ABIN8093192

The Rabbit Monoclonal anti-FOLR1 antibody is suitable to detect FOLR1 in samples from Human, Mouse, Rat and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for Recombinant FOLR1 antibody (Extracellular) (ABIN8093192)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FOLR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: FOLR1-13424R

Product Details anti-FOLR1 Antibody

Binding Specificity: AA 1-200, Extracellular

Purpose: FOLR1 Antibody / Folate receptor 1 (azide and preservative free)

Immunogen: A recombinant fragment (around amino acids 1-200) of human FOLR1 protein corresponding to the extracellular domain (exact sequence is proprietary) was used as the immuongen for the FOLR1/FR alpha antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the FOLR1/FR alpha antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: FOLR1/FR alpha antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: FOLR1

Background: FR alpha antibody is used to study Folate receptor alpha, a high-affinity folate binding protein that mediates receptor-dependent folate uptake at the cell surface. Folate receptor alpha is encoded by the FOLR1 gene and is anchored to the plasma membrane via a glycosylphosphatidylinositol linkage. This membrane association allows the receptor to concentrate folate at the cell surface and internalize it through endocytic pathways, supporting cellular processes that depend on one-carbon metabolism and nucleotide biosynthesis.

Folate receptor alpha is commonly referred to as FR alpha in the literature, particularly in studies focused on epithelial transport and cancer-associated folate metabolism. FR alpha expression is normally restricted to select epithelial tissues, where it localizes to the apical surface of polarized cells. This spatially regulated expression distinguishes FR alpha from other folate transport systems and has made it a useful marker for studying epithelial polarity, membrane trafficking, and nutrient uptake mechanisms. Use of a FR alpha antibody enables detailed examination of FR alpha distribution in tissue and cell-based models.

At the functional level, FR alpha facilitates uptake of folate by binding extracellular folate with high affinity and delivering it to intracellular compartments following endocytosis. This pathway operates independently of reduced folate carriers and is particularly important in environments where folate availability is limited. Studies using FOLR1 antibody have contributed to understanding how FR alpha-mediated transport supports proliferation and survival in rapidly dividing epithelial cells.

Aberrant expression of FR alpha has been widely documented in epithelial malignancies, most notably in ovarian carcinoma but also in subsets of lung, breast, and endometrial cancers. In these contexts, elevated FR alpha expression reflects altered metabolic demands and changes in epithelial differentiation. Detection of FR alpha using a FOLR1 antibody supports research into tumor-associated folate metabolism, epithelial lineage markers, and metabolic adaptation in cancer cells.

FR alpha antibody (clone FOLR1/13424R) is designed to detect Folate receptor alpha in research applications. Evaluation of FR alpha expression provides insight into folate transport pathways, epithelial cell organization, and disease-associated changes in nutrient uptake. Overall, Folate receptor alpha remains a key protein linking membrane transport, epithelial biology, and metabolic regulation.

UniProt: P15328

Pathways: Dicarboxylic Acid Transport