Isocitrate Dehydrogenase antibody

Catalog No. ABIN8093273

The Mouse Monoclonal anti-Isocitrate Dehydrogenase antibody is suitable to detect Isocitrate Dehydrogenase in samples from Human, Rat and Mouse. It has been validated for WB, IF, IHC (p) and FACS.

Quick Overview for Isocitrate Dehydrogenase antibody (ABIN8093273)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Isocitrate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 6B13

Product Details anti-Isocitrate Dehydrogenase Antibody

Purpose: Isocitrate Dehydrogenase 2 / IDH2

Sequence: KDLAGCIHGL SNVKLNEHFL NTTDFLDTIK SNLDR

Purification: Affinity purified

Immunogen: Amino acids KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR from the human protein were used as the immunogen for the Isocitrate Dehydrogenase 2 antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the Isocitrate Dehydrogenase 2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Isocitrate Dehydrogenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Alternative Name: Isocitrate Dehydrogenase 2 / IDH2

Background: Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

UniProt: P48735